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KMID : 0368419980410010064
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Journal of Plant Biology
1998 Volume.41 No. 1 p.64 ~ p.67
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The Effect of BAP on the Gene Expression of a Small GTP-Binding Protein, Rho1Ps in a Shoot Apex of Garden Pea
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Nam, Sang-June
Lee, Myeong-Min/Lee, Sun-Hi/Lee, June-Seung/Kim, Seong-Ki
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Abstract
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The effect of a synthetic cytokinin, 6-benzylaminopurine (BAP), on gene expression of RholPs in shoot apex segments of garden pea was examined by Northern blot hybridization using ^32P-labelled 560 bp partial RholPs cDNA fragment as the probe. The application of 10^(-12) M BAP, the lowest concentration tested, for 24h to the segments strongly activated the synthesis of RholPs transcripts as much as 7 times compared with those in BAP-untreated segments. In addition, the RholPs transcripts synthesis was enhanced by BAP in a concentration dependent manner, suggesting that BAP is concretely involved in the RholPs gene expression in the segments. A time course analysis of BAP on RholPs gene expression exhibited that BAP was closely related not only to the RholPs transcripts synthesis but also to the degradation of RholPs transcripts.
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